Protocols

The Mayo Clinic Brain Tumor Patient-Derived Xenograft National Resource provides information about techniques for establishing brain tumor PDX lines for research. Here, we explain our methodology for establishing PDX models, serial passaging, in vitro culture and more.

Mouse colony protocols

• Establishment, Restoration and Passaging of PDX Flank Tumor Lines
• Cryopreservation of PDX Flank Tumor Tissue
• Cell Harvest From PDX Flank Tumor

Experimental protocols

• Flank Tumor Implantation Using PDX Explant Cultures
• Intracranial Tumor Implantation Using PDX Explant Cultures
• In Vitro Culturing and Experimentation

Sample shipment and receipt protocols

• FedEx Label Protocol
• Cryomush (Cryopreserved Tissue) Implantation Instructions
• Cryopreserved PDX Cell Culture Instructions
• Fresh PDX Cell Culture Instructions
• PDX Tumor Extract Information
• TMZ Resistant Subline Culturing