Facilities and Equipment

Facilities

The Metabolomics Core facilities are divided into two locations at Mayo Clinic in Rochester, Minnesota:

• Mass spectrometry facilities. Mass spectrometry facilities occupy approximately 3,500 square feet in the Alfred Building at Mayo Clinic Hospital — Saint Marys Campus. The facilities comprise two instrument rooms, a work-up laboratory and office space, and a conference room.
• Nuclear magnetic resonance (NMR) facilities. NMR spectroscopy facilities include approximately 3,100 square feet in the basement of the Stabile building, housing instruments, offices and a wet lab.

Equipment

Mass spectrometry equipment

The Mayo Clinic Metabolomics Core has 14 mass spectrometers divided into two categories: single quadrupole and tandem instruments.

Single quadrupole instruments include:

• Agilent Technologies 5975C MSD
• Agilent Technologies 5977A MSD
• Agilent Technologies 5977B MSD

Tandem instruments include:

• Applied Biosystems Sciex 6500 (LC/MS/MS)
• Applied Biosystems Sciex 7500 (LC/MS/MS)
• Agilent Technologies 6460 (LC/MS/MS)
• Agilent Technologies 6550iFunnel Q-TOF (LC/MS/MS) x 2
• ThermoFisher Scientific TSQ Quantum Ultra (LC/MS/MS)
• ThermoFisher Scientific TSQ Quantiva (LC/MS/MS) x 2
• ThermoFisher Scientific TSQ Quantis Plus (LC/MS/MS)
• ThermoFisher Scientific Q-Exactive Plus (LC/MS/MS)

NMR spectroscopy equipment

The Mayo Clinic Metabolomics Core's NMR equipment includes:

• Bruker DRX-700 (700 MHz 1H, 16.4 tesla) 3-channel equipped with cryoprobe (5 mm triple resonance ¹H, ¹³C, ¹⁵N, proton detection, and single axis gradient), 5 mm broadband (¹⁵N to ³¹P, 51 to 209 MHz) with ¹H decoupling for proton and heteronuclear spectroscopy.
• Bruker Avance III 600 MHz spectrometer, a high-resolution ¹H NMR, with high-throughput sample handler for metabolomics studies.
• Bruker 500 MHz spectrometer (Avance) used for ¹⁸O-assisted ³¹P NMR studies of cellular phosphoryl-containing metabolites.