Facilities and Equipment
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Helios mass cytometer
The Standard BioTools Helios mass cytometer is a high-plex, single-cell cytometry system that uses cytometry by time-of-flight (CyTOF) technology to analyze cells. This system combines the front-end fluidics capabilities of a flow cytometer with the high-resolution signal detection capabilities of a mass spectrometer. This system detects heavy metal-labeled antibodies in the time-of-flight chamber of an inductively coupled mass spectrometer (ICP-MS). These capabilities allow the theoretical detection of 135 unique channels on single cells that are introduced into this system. High-parameter antibody panels can be designed and used to characterize single cells in suspension derived from blood and tissue.
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Akoya Phenocycler-Fusion
The Akoya Biosciences Phenocycler-Fusion system is a high-speed cutting-edge imaging system that couples an automated fluidics control system — Phenocycler — with a high-resolution image scanning system — PhenoImager. The combination creates highly accurate spatial multiomics fluorescent images that allow single-cell and subcellular structure analysis. This system has the potential to monitor more than 100 protein targets on a single slide using oligonucleotide conjugated antibodies and complementary fluorescent labeled probes. Cyclic hybridizations and scanning allow for multiple rounds of staining and acquisition of high-parameter multiplexed images.
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Hyperion imaging system
The Standard BioTools Hyperion imaging system provides a mass cytometry-based one micron resolution ablation system that allows multiplexed imaging using heavy metal-tagged antibodies. The Helios mass cytometer enables the sensitive and precise detection of metal-tagged antibodies bound to tissue regions of interest interrogated by the Hyperion tissue imager.
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Bio-Rad S3e Cell Sorter
This is an automated cell sorter with real-time monitoring. The system allows two-way cell sorting with an easy-to-use interface.
The Immune Monitoring Core is supported by the Mayo Clinic Comprehensive Cancer Center. The lab occupies 870 square feet on the fifth floor of the Guggenheim Building at Mayo Clinic's campus in Rochester, Minnesota, and 229 square feet in the Johnson Research Building at Mayo's campus in Scottsdale, Arizona.
The Immune Monitoring Core uses several multiparameter cytometry and imaging modalities to perform single-cell analysis studies, including Standard BioTools Helios cytometry by time-of-flight (CyTOF) technology. The core provides services using the Standard BioTools Hyperion Imaging System. Mass cytometry technology using Helios CyTOF enables a comprehensive understanding of cell phenotypes, signaling pathways and function. The core currently has three Helios systems, with two located in Rochester and one located at Mayo Clinic's facility in Scottsdale, Arizona. Helios also serves as a high-dimensional single-cell suspension analysis tool when decoupled from the Hyperion tissue imager.