Software
TractionsForAll v1.0 is a freely distributed program that calculates 2D tractions exerted by an adherent cell on its substrate. Dr. Tschumperlin's research team created it to allow students and researchers in the field of mechanobiology to study contractile responses of a variety of cell types.
Although helpful for understanding the process, no background in applied mechanics and computer programming is needed for using the software. The program is written and compiled in MATLAB, a matrix laboratory developed by MathWorks.
More details about the background theory and implemented experimental techniques can be found in the following references:
- Marinkovic A, Mih JD, Park JA, Liu F, Tschumperlin DJ. Improved throughput traction microscopy reveals pivotal role for matrix stiffness in fibroblast contractility and TGF-beta responsiveness. American Journal of Physiology — Lung Cellular and Molecular Physiology. 2012; doi:10.1152/ajplung.00108.2012.
- Mih JD, Sharif AS, Liu F, Marinkovic A, Symer MM, Tschumperlin DJ. A multiwell platform for studying stiffness-dependent cell biology. PLOS One. 2011; doi:10.1371/journal.pone.0019929.
- Butler JP, Tolic-Norrelykke IM, Fabry B, Fredberg JJ. Traction fields, moments, and strain energy that cells exert on their surroundings. American Journal of Physiology — Cell Physiology. 2002; doi:10.1152/ajpcell.00270.2001.
- Tolic-Norrelykke IM, Butler JP, Chen J, Wang N. Spatial and temporal traction response in human airway smooth muscle cells. American Journal of Physiology — Cell Physiology. 2002; doi:10.1152/ajpcell.00169.2002.