Inclusion Criteria:

• Age > 18 years old.
• ECOG performance status (PS) 0, 1 or 2.
• Clinical and radiographic evidence suggesting a diagnosis of a diffuse glioma, or a prior diagnosis of a diffuse glioma.
• Diffuse gliomas include diffuse astrocytoma, anaplastic astrocytoma, glioblastoma, oligodendroglioma and anaplastic oligodendroglioma.
• Planned neurosurgical procedure for purposes of biopsy or resection of suspected or previously diagnosed brain tumor as part of routine clinical care.
• Willing to undergo neurosurgical resection or biopsy at Mayo Clinic (Rochester, MN).
• Ability to understand and the willingness to sign a written informed consent document.

 Exclusion Criteria:

• Vulnerable populations:
  • Pregnant women;
  • Prisoners;
  • Mentally handicapped.
• Patients who are not appropriate candidates for surgery due to current or past medical history or uncontrolled concurrent illness.